pisum sativum psa Search Results


pisum sativum agglutinin  (Vector Laboratories)
93
Vector Laboratories pisum sativum agglutinin
Pisum Sativum Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pisum sativum agglutinin/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
pisum sativum agglutinin - by Bioz Stars, 2026-02
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pisum sativum agglutinin psa  (Vector Laboratories)
93
Vector Laboratories pisum sativum agglutinin psa
Pisum Sativum Agglutinin Psa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pisum sativum agglutinin psa/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
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biotinylated mabs  (Vector Laboratories)
93
Vector Laboratories biotinylated mabs
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Biotinylated Mabs, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated mabs/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
biotinylated mabs - by Bioz Stars, 2026-02
93/100 stars
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pisum sativum agglutinin (psa)  (EY Laboratories)
90
EY Laboratories pisum sativum agglutinin (psa)
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Pisum Sativum Agglutinin (Psa), supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pisum sativum agglutinin (psa)/product/EY Laboratories
Average 90 stars, based on 1 article reviews
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90/100 stars
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ar assay kit containing pisum sativum agglutinin (psa) labelled with fluorescein isothiocyanate (fitc) (psa-fitc)  (SAS institute)
90
SAS institute ar assay kit containing pisum sativum agglutinin (psa) labelled with fluorescein isothiocyanate (fitc) (psa-fitc)
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Ar Assay Kit Containing Pisum Sativum Agglutinin (Psa) Labelled With Fluorescein Isothiocyanate (Fitc) (Psa Fitc), supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ar assay kit containing pisum sativum agglutinin (psa) labelled with fluorescein isothiocyanate (fitc) (psa-fitc)/product/SAS institute
Average 90 stars, based on 1 article reviews
ar assay kit containing pisum sativum agglutinin (psa) labelled with fluorescein isothiocyanate (fitc) (psa-fitc) - by Bioz Stars, 2026-02
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fluorescein isothiocyanate-labelled pisum sativum agglutinin fitc-psa  (Blackwell Science Ltd)
90
Blackwell Science Ltd fluorescein isothiocyanate-labelled pisum sativum agglutinin fitc-psa
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Fluorescein Isothiocyanate Labelled Pisum Sativum Agglutinin Fitc Psa, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein isothiocyanate-labelled pisum sativum agglutinin fitc-psa/product/Blackwell Science Ltd
Average 90 stars, based on 1 article reviews
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fluorescein isothiocyanate conjugated to pisum sativum agglutinin (psa)  (ICN Biomedicals)
90
ICN Biomedicals fluorescein isothiocyanate conjugated to pisum sativum agglutinin (psa)
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Fluorescein Isothiocyanate Conjugated To Pisum Sativum Agglutinin (Psa), supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein isothiocyanate conjugated to pisum sativum agglutinin (psa)/product/ICN Biomedicals
Average 90 stars, based on 1 article reviews
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garden pea (pisum sativum) agglutinin (psa)  (Tokyo Chemical Industry)
90
Tokyo Chemical Industry garden pea (pisum sativum) agglutinin (psa)
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Garden Pea (Pisum Sativum) Agglutinin (Psa), supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/garden pea (pisum sativum) agglutinin (psa)/product/Tokyo Chemical Industry
Average 90 stars, based on 1 article reviews
garden pea (pisum sativum) agglutinin (psa) - by Bioz Stars, 2026-02
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fluorescein isothiocyanate (fitc) conjugated to pisum sativum agglutinin (psa)  (ICN Biomedicals)
90
ICN Biomedicals fluorescein isothiocyanate (fitc) conjugated to pisum sativum agglutinin (psa)
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Fluorescein Isothiocyanate (Fitc) Conjugated To Pisum Sativum Agglutinin (Psa), supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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pisum sativum agglutinin (psa  (Cosmo Bio USA)
90
Cosmo Bio USA pisum sativum agglutinin (psa
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Pisum Sativum Agglutinin (Psa, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pisum sativum agglutinin (psa/product/Cosmo Bio USA
Average 90 stars, based on 1 article reviews
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acrosomal content marker pisum sativum agglutinin coupled to fluorescein isothiocyanate (psa-fitc)  (Anhui Anke)
90
Anhui Anke acrosomal content marker pisum sativum agglutinin coupled to fluorescein isothiocyanate (psa-fitc)
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Acrosomal Content Marker Pisum Sativum Agglutinin Coupled To Fluorescein Isothiocyanate (Psa Fitc), supplied by Anhui Anke, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pisum sativum agglutinin- fluorescein isothiocyanate (psa- fitc) kit  (Genmed Inc)
90
Genmed Inc pisum sativum agglutinin- fluorescein isothiocyanate (psa- fitc) kit
Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of <t>biotinylated</t> MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated <t>MAbs</t> were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.
Pisum Sativum Agglutinin Fluorescein Isothiocyanate (Psa Fitc) Kit, supplied by Genmed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pisum sativum agglutinin- fluorescein isothiocyanate (psa- fitc) kit - by Bioz Stars, 2026-02
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Image Search Results


Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of biotinylated MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated MAbs were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.

Journal: Journal of Virology

Article Title: Topological Analysis of HIV-1 Glycoproteins Expressed In Situ on Virus Surfaces Reveals Tighter Packing but Greater Conformational Flexibility than for Soluble gp120

doi: 10.1128/JVI.01145-13

Figure Lengend Snippet: Ligand cross-competition on soluble gp120 and VLPs. MAb and sCD4 relationships were explored on soluble gp120 (A) and VLPs (B). Panels A and B are organized in identical fashions to facilitate comparisons. Data are expressed as percentages derived from the ratio of titers of biotinylated MAb (horizontal series) in the presence or absence of excess (10 μg/ml) unlabeled competitor (vertical series), wherein titers determined without competitor are taken as 100%. Colors are used to emphasize inhibition and enhancement. Thus, inhibitions are shown as warm colors and enhancements are shown in cyan and magenta. In cases where biotinylated MAbs were used in combination with sCD4 in a particular column, the reference control also included sCD4. Each datum is the average of at least two repeats and is presented as the nearest whole number. In most cases, SOS-VLPs were used for panel B. However, as indicated, other VLPs were used in some situations. For example, cells in which UNC VLPs were used are delimited by a blue box. Data that differ >3-fold between panels A and B, and in which one or the other datum point lacks competition or inhibition (i.e., is >30% and <200%), are bordered by black boxes. ND, not done. aThe mean effect was determined by averaging the horizontal numbers. bThe ratio of mean enhancements was determined by the following formula: mean percent effect on VLPs/mean percent effect on soluble gp120. The ratio of mean inhibitions was determined by the following formula: mean percent effect on soluble gp120/mean percent effect on VLPs. Ratios of <1 (dark blue shading) indicate a pronounced effect on soluble gp120, ratios of >1 (dark green) indicate a pronounced effect on VLPs, and ratios of 1.0 (white) indicate no significant competition differences between the two antigens.

Article Snippet: Biotinylated MAbs were detected using streptavidin-alkaline phosphatase (Vector, Burlingame, CA).

Techniques: Derivative Assay, Inhibition